Cabazitaxel-loaded poly(alkyl cyanoacrylate) nanoparticles: Toxicity and changes in the proteome of breast, colon and prostate cancer cells

Nanoparticles composed of poly(alkyl cyanoacrylate) (PACA) have shown great promise due to their biodegradability and high drug loading capacity. Development optimal PACA nanocarriers requires detailed analysis the overall cellular impact exerted by variants. We here perform a comprehensive comparison cabazitaxel (CBZ)-loaded three different monomers, i.e. poly(n-butyl (PBCA), poly(2-ethylbutyl (PEBCA) poly(octyl (POCA). The cytotoxicity drug-loaded empty nanoparticles were compared that free CBZ across panel nine cancer cell lines assessing metabolism, proliferation protein synthesis. analyses revealed all CBZ-loaded PACAs was similar for tested, whereas much lower toxicity. To increase our understanding toxic effects these treatments MS-based proteomics performed with HCT116, MDA-MB-231 PC3 cells incubated PACA-CBZ variants or CBZ. Interestingly, specifically led decreased levels proteins involved in focal adhesion stress fibers lines. Since we recently demonstrated encapsulation within PEBCA significantly improved therapeutic effect on patient derived xenograft model mice, investigated this variant more closely immunoblotting. detected several changes expression degree phosphorylation SRC-pathway can be relevant substances.